The casting was confirmed by the production team on 4 August and the star begins performances on 12 September.
She will play Roxie Hart, a narcissistic murderer obsessed with fame.
Pamela Anderson made her broadway debut in the role earlier this year.
The 41-year-old is the first trans woman to play Roxie and one of the first trans women to play a leading Broadway role.
Chicago is the longest running stage show in Broadway history and continues to make waves in the theatre industry with its inclusive casting.
Ross joins a handful of Black trans women who have set foot on the Broadway stage.
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This year, L Morgan Lee secured a Tony nomination for her performance in A Strange Loop.
Ross has an undoubtedly impressive back catalogue.
She is best known for her role as Candy Ferocity in Emmy-award winning series Pose. Candy is a young trans woman who often competed in New York’s ballroom scene.